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Antibody Affinity Maturation

10 - 50 fold Affinity Improvement

Affinity maturation is the process by which antibodies increased its affinity, avidity and activity through multiple rounds of somatic hyper mutation (SHM) in germinal centers. Successive generations of B cells mutate and present to the antigen, that recognize the antigen with high affinity will survive and the low affinity ones are eliminated. Affinity maturation process more often applied to antibody leads that are selected from library approaches using a phage display technique and the affinity of the leads have 10 –100 nM affinity levels to the target. In antibody drug discovery, affinity maturation is applied to antibody leads to increase the affinity at least 10 to 50 folds.

GenScript conduct affinity maturation according to the parent antibody sequence that client provided through FASEBA screening to increase the antibody affinity to target antigen at least 5 fold and it can go up to 100 folds. Our FASEBA (FASEBA (Fast Screening for Expression, Biophysical-properties and Affinity) is a patented E.Coli based system that selects good affinity, expression level and thermostable candidates. Affinity maturation turnaround time for this process would be 4 to 5 months.

FASEBA – Fast Screening Expression Biophysical-Properties and Affinity

FASEBA technology is used to select the best protein binders from a large number of protein candidates in a high throughput fashion, without actually purifying these proteins. FASEBA selection is based on the expression level, biophysical properties, Binding affinity, cell binding, and functional activity of protein candidates.

Antibody Affinity Maturation Service (Cat. No.: SC1979)

Service Includes Deliverable Timeline
Antibody sequencing and sequence analysis
  • Antibody sequence, report of sequence analysis
24 weeks
Library design and construction
  • Library plasmid and glycerol stock, Report of library development
Phage display and FASEBA screening
  • Top five Fab candidates; Report of screening
Antibody production and characterization
  • Final report and purified antibody

Please contact us mentioning "Discovery Biology", our customer service representatives are available 24 hours a day, Monday through Friday to assist you.