Bispecific Antibody Discovery Service

Knob into hole(KIH)

Mutate one Thr in the CH3 region of one H chain to Tyr, forming a prominent "Knobs" structure, while one larger Tyr residue in the CH3 region of the other H chain is mutated to a smaller Thr, forming a concave "holes" structure. The steric hindrance effect of this structure can achieve correct assembly between two different H chains.

CrossMab

Based on the "Knob into hole (KIH)" structure, the CH1 and CL of the Fab region are switched to reduce L chain mismatch.

IgG fused with scFv

Bispecificity is achieved by fusing two single-chain antibodies (scfv) to a normal IgG antibody. Pairs of scfv can be attached to the C-terminus or N-terminus of each L or H chain, and the specificity of each scfv can be the same or different.

DVD-Ig

The VL and VH domains of one Ab are attached to the N-terminus of each Fab arm of one normal IgG Ab respectively, resulting in a tetravalent molecule with two binding sites for each antigen. DVD-Ig can achieve bispecificity through two variable regions binding dual targets. This technique avoids mismatching of different H/L chains.

GenScript ProBio SMABody^TM(Single-domain antibody fused to mAb) platform combines naturally generated single domain antibody and monoclonal antibody(mAb) to form a bispecific antibody in symmetric format with good biological efficacy and good developability，it only needs 3-5 month for SMABody™ screening & evaluation.