Chinese
Antibody-drug conjugates (ADCs) empower therapeutic efficacy of monoclonal antibodies by combining with chemotherapeutic agents. Monoclonal antibodies act as the Trojan horse to deliver small molecular drugs, via binding to antigens (e.g. receptors) on the target cell surface, causing endocytosis and subsequently releasing the cytotoxic molecules. The antibody internalization assay is an potent approach to streamline ADC discovery and development. GenScript Probio offers an effective method to discover suitable antibody candidate for ADC development.
Figure1: Progress of antibody internalization
Antibodies coupled with pH-sensitive dye only show fluorescence when being internalized by the target cell in its endosome. Such a fluorescence signal can be quantitated using a flow cytometer.
Case 1: Comparison of the dose-response relationship between fluorescence signal and antibody concentration can reflect the antibody internalization effect curve. After optimization, GenScript ProBio can effectively expand the detection window and provide a stable dose-response curve with high signal-to-noise ratio. As the figure shows, the labeled Herceptin is internalized by SK-RB-3 cells, emitting bright fluorescence and clearly showing specific antibody internalization signals.
Case 2: S/N ≥10, it is easy to distinguish the candidate antibody molecules with different internalization ability of the same target. As the figure shows, comparison of internalization effect of different anti-Claudin 18.2 monoclonal antibody samples (A-D).