Endpoint-Based, and Speedy
Dramatic improvement of ADCC by using recombinant NK cells
Antibody therapy has been proven to be highly powerful for cancer treatment. Two important mechanisms used by antibody drugs to kill targeted tumor cells are Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC), and Complement Dependent Cytotoxicity (CDC). GenScript ProBio is pleased to present to you both a PBMC-based ADCC assay and natural kill cell-based ADCC assay. The readout is endpoint-driven (target cell lysis). The CDC assay uses normal human serum as the source of complement. By implementing strict QC standards, GenScript ProBio can provide the assurance of the efficacy and potency profiles of your therapeutic antibodies.
Figure 1 Concentration-dependent study of Herceptin-mediated ADCC on SK-BR-3 cells by using PBMC as effector cells (human IgG as negative control)
Figure 2 Concentration-dependent study of Erbitux-mediated ADCC on A431 cells by using freshly isolated NK cells as effector cells (Rituxan as negative control)
Figure 3 Concentration-dependent study of Humira-mediated ADCC on CHO/tm TNF-α cells by using PBMC as effector cells (human IgG as negative control). CHO/tm TNF-α was a cell line stably expressed membrane bound TNF-α at which the proteolytic cleavage site was modified.
NHS: normal human serum