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Host Cell Commercial License

CHOK1-GenS
Cell Line Development System Commercial License

GenScript ProBio is now licensing the CHOK1-GenS cell line development system to biopharma companies for commercial use. Contact us for more information!

GenScript ProBio has 12 years of experience in mammalian cell line development, and is one of the earliest companies getting the CHO-K1 commercial use license and sublicense agreement from ECACC.

After serum-free suspension adaption and characterization, GenScript ProBio acquires the host cell for CHOK1-GenS cell line development system without chemical modification. In together with the high-expression proprietary pGenHT 1.0-DGV vector, the yield titer can reach an average of 4.35g/L , up to 8.70g/L

GenScript ProBio is experienced with 300+ cell line development projects, and the CHOK1-GenS system has been used in 30+ CMC&CMO projects in which 5 has got IND approvals.

CHOK1-GenS system exclusive advantages

  • system exclusive advantages
  • system exclusive advantages

High productivity with CHOK1-GenS system

  • High antibody titerHigh antibody titer - average titer: 4.35g/L, up to 8.70g/L

    CHOK1-GenS system
  • Suitable for different type of moleculesSuitable for different type of molecules

    CHOK1-GenS system

Excellent stability with CHOK1-GenS system

  • No. Type Study period T1(g/L) Titer variation
    1 IgG 1 PCB-60days 3.43 -9.90%
    2 IgG 1 PCB-60days 3.54 -7.00%
    3 IgG 4 PCB-50days 3.15 -9.00%
    4 IgG 4 PCB-60days 3.63 -8.20%
    5 IgG 4 PCB-60days 4.23 -10.40%
    6 Bispecific mab PCB-60days 2.72 -3.40%
    7 IgG 1 PCB-60days 4.68 -22.22%
    8 IgG 1 PCB-96days 4.76 -23.39%
    9 Protein PCB-50days 1.91 -11.52%
    10 IgG 1 PCB-60days 6.37 -4.55%
    11 tri-specific ab PCB-50days 0.34 -17.60%
    12 IgG 1 WCB-50days 3.78 -24.60%
    13 IgG 1 WCB-50days 5.74 -20.55%
    • The titer at the end of stability study during fed-batch is recorded as T2.
    • The titer at the beginning of stability study during fed-batch is recorded as T1.
    • Titer variation=(T2-T1)/T1*100%
    • Titer variation should be within 30%.

High productivity with CHOK1-GenS system

  • Titer prior to cell cultureTiter prior to cell culture up to 8.70 g/L

    High productivity with CHOK1-GenS system
  • Excellent Stability in Scale-UpExcellent Stability in Scale-Up from 3L to 200L

    • Excellent Stability in Scale-Up1
    • Excellent Stability in Scale-Up2
    • Excellent Stability in Scale-Up3
    • Excellent Stability in Scale-U3

CHOK1-GenS delivery package

Delivery Package Delivery items
Host system CHOK1-GenS cell line
Proprietary pGenHT 1.0-DGV vector
Full Traceability Documentation ECACC license agreement and original invoice
Adaptation report
cGMP cell banking report
cGMP cell bank testing report
Vector synthesis report
Vector map and key elements description
Detailed Protocols and Guidance Cell culture protocol
Clone strategy to help construct plasmid correctly
(enzyme cleavage used for gene of interest clone and plasmid linearization)
Cell line development protocol to screen cell clone with the host cell and vector
(including transfection, cell pool screening and cell clone screening)
Technical Support Comprehensive free technical support to ensure your success