Assay cell lines are used in a variety of applications, including gene functional studies, binding/blocking screening assay, and cross-species binding screening assay during antibody development. GenScript ProBio possesses a series of assay cell line construction technologies and is extensively experienced in mammalian cell culture and gene-editing, which enables the construction of a variety of assay cell lines and functional reporter assay cell line through CRISPR, Tet-On technology, lentivirus infection, electroporation, and lipo-transfection, thus shortening your project turnaround time.
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Lentivirus infection: Lentivirus can infect suspension cells and primary cells.
Non-lentivirus infection: Electro-transfection and nuclear-transfection are utilized for lentivirus-sensitive cell lines
GenScript ProBio has successfully constructed more than 500 assay cell lines, of which more than 100 can be used for antibody development, such as PD-1, PD-L1, Tim3, CTLA4, Lag3, Siglec15 as well as GPCR cell lines.
Part of the assay cell lines developed and available for antibody drug development
1.One-stop service: Service package including gene synthesis, vector construction, lentivirus packaging, and host cell characterization to ensure the delivery of cell lines with stable expression of genes inserted. All we need is your gene name of interest.
2.Proof of single cell clonality: Molecular Devices CloneSelect Imager imaging system verifies the monoclonality of cells.
3.Customized construction of expression vectors: Multiple promoters or co-expression of two or more genes in one plasmid. Various promoters and tags are available for your choice.
4.Multiple choices of expression validation methods: qPCR, RT-PCR, ELISA, Immunofluorescence, FACS and Western Blot.
5.Quality check: Delivered cell line free of fungus, bacteria and mycoplasma with viability over 90%.
6.Quality assurance: traceable experiment records.
7.Short turnaround time: Single cell clones delivered in 10-15 weeks.
A common problem when developing assay cell lines is that the protein expression level is relatively low but mRNA level is high. We tackled this problem by co-expressing suitable co-factor or chaperone proteins which had the potential improve the expression level by 1000 folds.
Targeting a specific protein isoform is challenging in antibody development due to high sequence identity between these isoforms. By generating assay cells expressing different protein isoforms, antibodies’ specificity can be validated using FACS binding assay. For instance, anti-Claudin 18.2 antibody screening was incorporated with a counter-screen against Claudin 18.1, which is ubiquitously expressed in normal tissue.
Anchoring secreted proteins on a cellular surface is a common way to enable antibody screening. One example is to construct an assay cell line expressing pro-inflammatory cytokine, tumor necrosis factor-α (TNF-α), which is usually secreted by macrophages.
*The timeline is for estimation only, the actual delivery time may vary due to the complexity of biological process.