Home » Biologics Discovery » Pharmacology & Discovery Reagent Service » In Vitro Pharmacology » Pseudovirus Neutralization Assay Services for SARS-CoV-2

Workflow of Neutralization Assay

Service Highlights

  • Virus entry assay model:
    A viral entry model, mimicking physiological entry mechanism.
  • Variants of SARS-CoV-2 available
    Extensible system, SARS-CoV-1 and multiple variants available, including Omicron; customized variant available.
  • High specificity:
    Ability to specifically screen drugs blocking pseudovirus entry.
  • High sensitivity:
    S/N > 100, activities of different candidates can be clearly distinguished.
  • Fast delivery:
    1-3 weeks to get results

Service Details

Starting materials
  • ≥ 100 μg neutralizing antibody for single dose screening, or ≥ 200 μg for dose-response assay
  • supernatant with antibody ≥ 1 μg/mL, more than ≥ 500 μL (Optimization is needed for different culture media)
  • Serum samples from immunized animals. Human samples should be tested with pseudovirus neutralization kit
  • Small molecules, peptides or other candidate samples inhibiting virus entry
  • Pseudovirus and target cells for infection are provided by GenScript ProBio

Delivery: Neutralization assay report

QC standard: S/N ≥ 100

Turnaround time:  1-3 weeks*

*TAT may extend due to the number of samples.

Pseudovirus Neutralization Assay Kit

Product Cat. No. name Deliverable TAT
Luciferase reporter kit SC2087A SARS-CoV-2 Pseudovirus Neutralization Assay kit_Luc reporter

    Components:

  • R Pseudovirus:
  • 1 vial of Luc reporter or 2 vials of GFP reporter for a 96-well plate
  • 10^7-8 IFU
  • R Target cell:
  • 2 vials for a 96-well plate
  • 2 × 10^6 cells/vial (proliferation-incompetent)
  • R Positive neutralizing antibody for 1 dose curve:
  • for 1 dose curve

Wild type:4 weeks

Mutant type:Quote

SC2087B SARS-CoV-2 D614G Pseudovirus Neutralization Assay kit_Luc reporter
SC2087C SARS-CoV-2 V367F Pseudovirus Neutralization Assay kit _Luc reporter
SC2087D SARS-CoV-2 W436R Pseudovirus Neutralization Assay kit _Luc reporter
SC2087E SARS-CoV-2 D364Y Pseudovirus Neutralization Assay kit _Luc reporter
SC2087K SARS-CoV-2 N501Y (B.1.1.7) Pseudovirus Neutralization Assay kit _Luc reporter
SC2087L SARS-CoV-2 N501Y (B.1.351) Pseudovirus Neutralization Assay kit _Luc reporter
SC2087M SARS-CoV-2 A222V Pseudovirus Neutralization Assay kit _Luc reporter
SC2087N SARS-CoV-2 N439K Pseudovirus Neutralization Assay kit _Luc reporter
SC2087O SARS-CoV-2 S477N Pseudovirus Neutralization Assay kit _Luc reporter
SC2087P SARS-CoV-2 E484K Pseudovirus Neutralization Assay kit _Luc reporter
SC2087Q SARS-CoV-2 Q677H Pseudovirus Neutralization Assay kit _Luc reporter
SC2087R SARS-CoV-2 Y453F & Δ69-70 Pseudovirus Neutralization Assay kit _Luc reporter
SC2087S SARS-CoV-2 N501Y Pseudovirus Neutralization Assay kit _Luc reporter
SC2087T SARS-CoV-2 E484K & N501Y (B.1.1.7) Pseudovirus Neutralization Assay kit _Luc reporter
SC2087U SARS-CoV-2 L452R (B.1.617.1) Pseudovirus Neutralization Assay kit _Luc reporter
SC2087V SARS-CoV-2 L452R (B.1.617.2) Pseudovirus Neutralization Assay kit _Luc reporter
SC2087W SARS-CoV-2 L452R (B.1.617.2-CDC, Delta) Pseudovirus Neutralization Assay kit _Luc reporter
SC2087X SARS-CoV-2 L452R (AY.1, Delta plus) Pseudovirus Neutralization Assay kit _Luc reporter
SC2087Y SARS-CoV-2 L452Q (C.37, Lambda) Pseudovirus Neutralization Assay kit _Luc reporter
SC2087Z SARS-CoV-2 B.1.621 (Mu) Pseudovirus Neutralization Assay kit _Luc reporter
SC2087-027 SARS-CoV-2 B.1.1.529 (Omicron) Pseudovirus Neutralization Assay kit _Luc reporter 
GFP reporter kit SC2087F SARS-CoV-2 Pseudovirus Neutralization Assay kit _GFP reporter
SC2087G SARS-CoV-2 D614G Pseudovirus Neutralization Assay kit _ GFP reporter
SC2087H SARS-CoV-2 V367F Pseudovirus Neutralization Assay kit _ GFP reporter
SC2087I SARS-CoV-2 W436R Pseudovirus Neutralization Assay kit _ GFP reporter
SC2087J SARS-CoV-2 D364Y Pseudovirus Neutralization Assay kit _ GFP reporter

* Components can be purchased separately. Please contact us.

Service Highlights

  • Virus entry assay model:
    A viral entry model, consistent with the physiological entry mechanism
  • Variants of SARS-CoV-2 available:
    Extensible system, SARS-CoV-1 and multiple variants available; cutomized variants available
  • High specificity:
    Ability to specifically screen drugs blocking pseudovirus entry
  • High sensitivity:
    S/N as high as about 100, activities of different candidates can be clearly distinguished
  • Ready to use:
    Save time on condition optimization
  • Free from sample degeneration:
    Especially for serum samples
  • Pseudovirus neutralization assay

  • Live virus neutralization assay

  • Figure 1. Comparable IC50 values of live virus and pseudovirus neutralization assay using the same sample.

  • Neutralization by ACE2-Fc

    Figure 2. Positive control ACE2-Fc can effectively inhibit pseudovirus entry in a dose-dependent manner

  • Consistent IC50 in independent tests

    Figure 3. Antibody candidates were screened for their ability to inhibit pseudovirus entry.

  • Neutralization by immunized mice serum

    Figure 4. Immunized mouse serum can effectively inhibit pseudovirus entry

  • Figure 5. B.1.617.2 pseudovirus (Delta variant) was efficiently blocked by ACE2-Fc chimera proteins (left panel). RBD-63 mAb failed to inhibit the B.1.617.2 pseudovirus (Delta variant) infection (right panel).

  • Figure 6. Blocking Omicron pseudovirus entry into ACE2 overexpressing target cells by means of ACE2-Fc chimera protein.