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Human Tumor Cell Line Profiling

Wide range of cost-efficient, high throughput assays

Over 240 human tumor cell lines, including in vivo validated xenograft cell lines

Human Tumor Cell Line Profiling

Oncology drug discovery requires knowing the pharmacological mechanism, and efficacy of small molecules on human tumor cell lines. GenScript ProBio offers a comprehensive oncology drug discovery platform which includes antibody drug discovery, in vitro and in vivo pharmacology services. As part of our in vitro oncology services, the cost-efficient human tumor cell line profiling packages, OncoProfiler and XenoProfiler, provide you with a combined panel of over 240 human tumor cell lines as well as a range of high throughput screening (HTS) options. XenoProfiler is a panel of tumor cell lines that have been validated in vivo with xenograft models. Together, OncoProfiler and XenoProfiler not only provides efficacy data in human tumor cell lines, but can ensure translation of efficacy from in vitro to in vivo models.

Key Features

  • High quality assay criteria and short turnaround time
  • OncoProfiler includes 200 human tumor cell lines. XenoProfiler includes 40 cell lines, which have been validated in vivo with xenograft models
  • High throughput screening (HTS) options available (see below)

Tumor Cell Line Profiling Assays

As a fully custom service, we offer a wide range of assays to use for proliferation and apoptosis profiles. Additionally, we offer biomarker, cytokine induction and combinatorial drug effect screening.

Profile Assay Description Throughput
Proliferation
Luminescent cell viability assay
384-well
MTT/MTS assay
96-well
Cytokine induction 
Cell viability assay with optimized incubation time
96-well
Combinatorial drug effect
Using any of the above assays
Custom
Customer defines assay type for any of the profiles

Case study: Analysis of proliferation inhibition

Figure 1: Profile of three different compound effects on proliferation of 225 tumor cell lines

Dose response IC50 of three compounds are reported in the above graph against 225 different human tumor cell lines. Luminescence-based proliferation assay was used to profile compound effect.

Figure 2: Data reproducibility confirmed across plates

Doxorubicin was used as an internal reference compound. Shown above is the IC50 of doxorubicin on proliferation of BEL-7402 cells.

Figure 3: Full dose response data on the effect of doxorubicin on BEL-7402

Doxorubicin dose response on inhibition of proliferation of BEL-7402 is shown above.

Related services

  • ADCC & CDC assays – Screen antibody candidates for anti-tumor activity in vitro using NK-92 recombinant cells in an endpoint-based assay
  • Cellular Western assay – Get accurate dose response curves from a cell based assay. Available in 96 or 384 well format, with two near-infrared probes

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