Entry of Coronavirus into host cells is mediated by the trimeric transmembrane spike (S) glycoprotein (Fig. 1). S glycoprotein is surface-exposed and mediates entry into the host cells by binding to human angiotensin-converting enzyme 2 (ACE2), the SARS-CoV-2 receptor, which makes S glycoprotein the major target of neutralizing antibodies and the focus of therapeutic and vaccine design.
A model that simulates the infection of the SARS-CoV-2 is designed by replacing the envelope glycoprotein in the lentivirus vector with the S protein. Neutralizing antibodies blocking S protein (Fig. 2). and receptor binding could prevent the virus from infecting host cells. By detecting the luminescence of target cells, it is possible to screen or verify the activity of the neutralizing antibody.
GenScript ProBio provides not only assay service but also neutralization kit, meeting all your needs for both research and industry application.
Fig.1 Pseudovirus with S glycoprotein as the envelope protein
Fig.2 Neutralizing antibodies preventing the pseudovirus from entering host cells
Delivery: Neutralization assay report
QC standard: S/N ≥ 100
Turnaround time: 1-3 weeks**TAT may extend due to the number of samples.
|Luciferase reporter kit||SC2087A||SARS-CoV-2 Pseudovirus Neutralization Assay kit_Luc reporter||
Wild type:4 weeks
|SC2087B||SARS-CoV-2 D614G Pseudovirus Neutralization Assay kit_Luc reporter|
|SC2087C||SARS-CoV-2 V367F Pseudovirus Neutralization Assay kit _Luc reporter|
|SC2087D||SARS-CoV-2 W436R Pseudovirus Neutralization Assay kit _Luc reporter|
|SC2087E||SARS-CoV-2 D364Y Pseudovirus Neutralization Assay kit _Luc reporter|
|GFP reporter kit||SC2087F||SARS-CoV-2 Pseudovirus Neutralization Assay kit _GFP reporter|
|SC2087G||SARS-CoV-2 D614G Pseudovirus Neutralization Assay kit _ GFP reporter|
|SC2087H||SARS-CoV-2 V367F Pseudovirus Neutralization Assay kit _ GFP reporter|
|SC2087I||SARS-CoV-2 W436R Pseudovirus Neutralization Assay kit _ GFP reporter|
|SC2087J||SARS-CoV-2 D364Y Pseudovirus Neutralization Assay kit _ GFP reporter|
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Pseudovirus neutralization assay
Live virus neutralization assay
Figure 1. Comparable IC50 values of live virus and pseudovirus neutralization assay using the same sample.
Neutralization by ACE2-Fc
Figure 2. Positive control ACE2-Fc can effectively inhibit pseudovirus entry in a dose-dependent manner
Consistent IC50 in independent tests
Figure 3. Antibody candidates were screened for their ability to inhibit pseudovirus entry.
Neutralization by immunized mice serum
Figure 4. Immunized mouse serum can effectively inhibit pseudovirus entry