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GenScript ProSpeed™
Single B Cell Screening Service

Screening in 24 hours

400 fold Higher Resolution

Brief Introduction of Single B Cell Screening Platform

Single B cell screening platform, also known as single B cell cloning technology, is a microsystem based screening method. This platform conducts isolation, screening and evaluation on the B cells, avoiding the cell fusion and the library construction step. Also, single B cell screening platforms usually integrate high throughput platforms, therefore assays could be carried out in a highly efficient manner.

GenScript’s ProSpeed™ Single B Cell Screening Service

GenScript has built our own single B cell platform by using Beacon™, a micro-chamber based platform. With dozens years of experience on antibody discovery and cell manipulation, GenScript now provides a one-stop solution on antibody discovery using single B cell screening technology.

Workflow

Service Features

  • EFFICIENCY: 3 Months → 24 Hours

    • Throughput: 11,000 & 14,000 nanopens/chip
    • Automation: From import, screening to export, reduced chance of human error
    • Assay results in minutes
  • MULTIPLE TYPES of ASSAY

    • In depth characterizations: Affinity, Specificity, and Functionality
    • Compatible for Protein-based & Cell-based assay

By isolating, confining, and interrogating each individual B cells, single B cell screening maximizes the antibody repertoires that we can access, therefore it is inherently superior in preserving the B cell diversity. The effective concentration in the microsystem allows the detection for antibody secretion from a single cell within minutes. This means using the single B cell screening platform, lead clones can be identified in less than 1 day. This platform can also be coupled with sample sources from recovered patients PBMC or express immunization of fully human transgenic mice, allowing you to obtain fully human antibody in 1 month! In Feb. 2020, our single B cell screening platform has shown to be an indispensable tool for identifying several promising leads against the 2019 NCP within 12 hours. See our Case Study

Case Study

  • Case Study
    • 122 bead binder

      122 bead binder

    • 95 cell binder

      95 cell binder

    • 35 cross reactive to cyno antigen

      35 cross reactive to cyno antigen

    • 82 mapped to a different epitope than a positive control antibody

      82 mapped to a different epitope than a positive control antibody

The flow chart shown the results of one of our case study. 8058 single B cell isolated in one chip, 122 of which are antigen specific binders, and 95 of the binders shows activity of cell surface binding. the binders showing cross reactivity with cyno (35) was collected for single cell sequencing.

Based on the mechanism and process of B cell isolation, there are multiple choices on the market to achieve single B cell screening. But for better using it in antibody drug discovery, accuracy, assay capability and sensitivity are the key issues that we need to consider. Here at GenScript, benefited from the OEP system of Beacon™ with the built-in analytical software, we can achieve the precise control and accurate analysis of each single cell, plus our assay versatility, we can deliver you the desired antibody candidates in 1 day!

Comparison of Single B Cell Screening Platforms

Sorting Platform Throughput Accuracy Automation Sensitivity Assay Capability
Multiple test Cell based assay
FACS Medium Low Low Low Y N
Microdroplets High Low Medium High Y N
Microengraved
micro/nanowells
Medium Medium Low High N N
Microfluidic Chamber
(Beacon™)
Sufficient High High High Y Y

Package of Genscript single B platform service

Throughput: 2 chips, leading to more binders, especially suitable for challenging targets.

Phase Item TAT
Material preparation Protein labelling
Cell validation (FACS)
Immunization Option 1: Immunization with protein (regular animals) 8-10 Weeks
Option 2: Immunization with protein (Humanized mouse) 8-14 Weeks
Assay on Single B System: Beacon
(2 chips)
Required Bead-based IgG 1-2 Weeks*
Bead-based antigen specific binding
Optional Cell based epitope mapping against 1 benchmark
Cell based Ag binding assay
Cell based cross species
Cell-based ligand blocking
Bead-based multiplex capture assay (e.g. cross species reactivity, extra antigen binding)
Sequencing Desirable single B cells (given the selection criteria) will be exported for total cDNA recovery and single cell VH/VL sequencing 1 Week
Recombinant Antibody Production Ab expression & purification 1-2 Weeks

*All assays are finished within 1 day. The rest of time is used for data processing and communication with clients.

Throughput: 2 chips

Phase Item TAT
Material preparation DNA plasmid synthesis
DNA validation & Gene bullet preparation
Protein labelling
Cell validation
Immunization Option 1: Immunization with DNA (regular animals) 10-12 weeks
Option 2: Immunization with cell (regular animals)
Option 3: Immunization with DNA & cell (regular animals)
Assay on Single B System: Beacon
(2 chips)
Required Bead-based IgG 1-2 Weeks*
Cell based Ag binding
Optional Cell based epitope mapping against 1 benchmark
Cell based cross species
Cell-based ligand blocking
Sequencing Desirable single B cells (given the selection criteria) will be exported for total cDNA recovery and single cell VH/VL sequencing 1 Week
Recombinant Antibody Production (Optional) Ab expression & purification 1-2 Weeks

*All assays are finished within 1 day. The rest of time is used for data processing and communication with clients.

Service flowchart

Service flowchart



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