A1. Affinity ranking of antibodies by BIAcore.
A2. Affinity determination of clone 2 antibody in different concentration (nM)
A. GenScript ProBio can not only provide mouse anti-ID antibodies, but also provide rabbit anti-ID antibodies that usually have a high affinity at the picomolar.
B. Blocking assay
B. Competitive ELISA with different concentration of drug target could set as an optional step to distinguish the type of anti-ID antibodies. Ab1 and Ab2, which could bind to the antibody drug when competed with drug target, are non-blocking anti-ID antibodies, while Ab3 and Ab4 are antigen-blocking antibodies.
C. Sandwich ELISA could be performed to select anti-ID pair which could largely increase the success rate of immunoassay development. As it was shown in the table, mAb2 is able to pair with other four clones (mAb1, mAb3, mAb4, mAb5).
D. After the antibody sensitivity analysis, the limit of detection (LOD) of the anti-idiotype Ab is typically less than 0.2ng/ml.
A. Affinity-purified anti-idiotype polyclonal antibodies showed specific binding to antibody drug. The cross rate with isotype control is less than 2%.
B. After the antibody sensitivity analysis, the limit of detection (LOD) of the anti-idiotype pAb is typically less than 10ng/ml.
The antibody is generally stable for more than 5 years by accelerating the stability experiment, covering the time of the customer's clinical declaration process.