300+ ab humanization projects
30+ affinity maturation projects delivered
“We are pleased with the antibody humanization service and the purification service of GenScript ProBio. It is very impressive and professional. we validated the humanization service by testing the antibody against anti-human IgG as the secondary antibody. The humanized antibody purification met our expectations and need. The characterization of the antibody that was highlighted by the COA is excellent. We would continue to avail your service and will also recommend it to anyone who needs such service. Thanks.” – Govind Panchamoorthy, Director, Genus Oncology
Immunogenicity can potentially lead to reduced efficacy through rapid clearance or neutralization of the drug, or toxicity due to cross-reaction with proteins in the body, which can have catastrophic consequences for the patient. Antibody humanization & antibodies characterization can reduce the immunogenicity of monoclonal antibodies derived from xenogeneic sources and to improve their activation of the human immune system by replacing non-human antibody frameworks with human ones. It is a very important step in therapeutic antibody discovery process.
A common method for humanization of non-human antibodies is complementary determining regions (CDR) grafting in which the CDRs of non-human antibodies are grafted onto the human frameworks. But only CDR grafting is not enough to get eliminate the risk of immunogenicity very well. Usually, back mutation will be performed to further transfer CDRs into human frameworks as similar as possible. In this step PTM hot spot analysis can be applied to increase the probability to get humanized antibody with least affinity loss and normal function.
Also, FASEBA(Fast Screening for Expression, Biophysical-properties and Affinity) high throughput screening can be applied to screen out humanized antibody with best affinity.
GenScript ProBio provides 2 packages for antibody humanization service. Deluxe package using CDR-grafting, back mutation with PTM analysis and screening with FASEBA which can guarantee no affinity loss for the humanized antibody. Express package using CDR grafting and back mutation to generate humanized antibody as fast as 6 weeks.
When using fluorophores, individually or in a multiplex format, it is important to pay attention to a few technical features including:
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