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Anti-idiotype Antibody and Immunoassay Development Services

14 years' experience with over 400 Projects

Guarantee application in immunoassay development for PK and ADA analysis

Applied as critical reagent in IND filing and clinical trials

Upgraded! Anti-idiotype Antibody Services

An anti-idiotype antibody (anti-ID Ab, see the schematic diagram) binds to the idiotype of another antibody, usually an antibody drug. An idiotype can be defined as the specific combination of idiotopes present within an antibodies complement determining regions. Since anti-ID Abs are capable of binding to antibody drugs within biological fluids, they are commonly used in preclinical setting for antibody drug pharmacokinetics (PK) and pharmacodynamics (PD). Due to the similarity between anti-ID Abs and anti-drug antibodies, anti-ID Abs are also commonly used as a reference standard for antibody drug immunogenicity, anti-drug antibody (ADA) studies and neutralization antibody measurement.

GenScript ProBio has 14 years experience in developing of highly sensitive and specific polyclonal and monoclonal anti-ID Abs to support your antibody drug development. We try to work as closely as possible with our customers to understand their specific downstream application in order to generate their perfect anti-ID. See our case studies »

Anti-Idiotype Antibodies

Type	1. Antigen-blocking	2. Non-blocking	3. Complex-specific
Schematic diagram
Feature	Paratope-specific	Not Paratope-specific	Partially paratope-specific
Antigen binding	Inhibitory	Not inhibitory	Not inhibitory
Application	Detect free drug only	Detect total drug (free and bound)	Detect bound drug typically
Selection Guideline	Type 1 or type 2 could be applied to detect antibody drug when having a cell-bound drug target e.g. PD1/PDL1 Type 1 & type 2 may both be needed when having a secret drug target e.g. TNF

Features of GenScript ProBio Anti-ID Antibodies Service

Application tailored anti-id mAb

Fit for capture ELISA & sandwich ELISA
Fast delivery

8 weeks to delivery purified Anti-id pAb with guaranteed quantity
High success rate

100% for blocking anti-id Ab

Anti-ID Package Details

Anti-id pAb Quantity Guaranteed Package

Typical Application	Starting material	Turnaround time (weeks)	Deliverable
Positive control for ADA mensuration	≥ 45 mg Target antibody drug (customer provide, purity > 85%) ≥ 30 mg Isotype control-human IgG (GenScript ProBio provide, purity > 90%)	Human IgG1: 8-10 Human IgG2/IgG4: 10-12 Other Type^#: >13	Small scale affinity purification after 2rd immunization for in-house detection (optional) Affinity purified pAb (at least 5 mg) COA report Pilot sensitivity report (optional) guarantee sensitivity>10ng/ml QC standard: ELISA titer ≥ 1: 729,000; cross rate ≤ 2%;Stability: >5 years

Typical Application

Starting material

Turnaround time (weeks)

Deliverable

Positive control for ADA mensuration

≥ 45 mg Target antibody drug (customer provide, purity > 85%)
≥ 30 mg Isotype control-human IgG (GenScript ProBio provide, purity > 90%)

Human IgG1: 8-10
Human IgG2/IgG4: 10-12
Other Type^#: >13

Small scale affinity purification after 2rd immunization for in-house detection (optional)
Affinity purified pAb (at least 5 mg)
COA report
Pilot sensitivity report (optional)
guarantee sensitivity>10ng/ml

QC standard: ELISA titer ≥ 1: 729,000; cross rate ≤ 2%;Stability: >5 years

^#Other type: including but not limited to peptide, protein, ADC, SvFv & bispecific antibody.

Anti-id mAb Golden Package for Capture ELISA

Typical application	Starting material	Turnaround time (weeks)	Deliverable
PK and neutralization Ab analysis	≥ 2 mg target antibody drug (customer provide, purity > 85%) 0.5 mg Isotype control or human IgG (GenScript ProBio provide, purity > 85%)	21-27 (Regular) 13-14 (Express)	1-2 mL supernatant/parental clone 5 mL final supernatant/subclone 3-5 Hybridoma cell lines 3-5 purified Ab, 2-5 mg per Ab Pilot sensitivity report (optional) guarantee sensitivity>10ng/ml COA report QC standard: ELISA titer ≥ 1: 729,000;Stability: >5 years

Typical application

Starting material

Turnaround time (weeks)

Deliverable

PK and neutralization Ab analysis

≥ 2 mg target antibody drug (customer provide, purity > 85%)
0.5 mg Isotype control or human IgG (GenScript ProBio provide, purity > 85%)

21-27 (Regular)

13-14 (Express)

1-2 mL supernatant/parental clone
5 mL final supernatant/subclone
3-5 Hybridoma cell lines
3-5 purified Ab, 2-5 mg per Ab
Pilot sensitivity report (optional)
guarantee sensitivity>10ng/ml
COA report

QC standard: ELISA titer ≥ 1: 729,000;Stability: >5 years

Guarantee to deliver one type of anti-id mAb (blocking or non-blocking)

Anti-id mAb Deluxe Package for Sandwich ELISA

Typical application	Starting material	Turnaround time (weeks)	Deliverable
PK and neutralization Ab analysis	≥ 2 mg target antibody drug (customer provide, purity > 85%) 0.5 mg Isotype control or human IgG (GenScript ProBio provide, purity > 85%)	21-27 (Regular) 13-14 (Express)	1-2 mL supernatant/parental clone 5 mL final supernatant/subclone 5-10 Hybridoma cell lines 5-10 purified Ab/clone, 2-5 mg/clone Anti-id mAb pair and pilot sensitivity report (optional) guarantee sensitivity>10ng/ml COA report QC standard: ELISA titer ≥1: 729,000;Stability: >5 years

Typical application

Starting material

Turnaround time (weeks)

Deliverable

PK and neutralization Ab analysis

≥ 2 mg target antibody drug (customer provide, purity > 85%)
0.5 mg Isotype control or human IgG (GenScript ProBio provide, purity > 85%)

21-27 (Regular)

13-14 (Express)

1-2 mL supernatant/parental clone
5 mL final supernatant/subclone
5-10 Hybridoma cell lines
5-10 purified Ab/clone, 2-5 mg/clone
Anti-id mAb pair and pilot sensitivity report (optional)
guarantee sensitivity>10ng/ml
COA report

QC standard: ELISA titer ≥1: 729,000;Stability: >5 years

Guarantee to deliver two types of anti-id mAb (blocking & non-blocking)
Guarantee to deliver at least one anti-id mAb pair

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

Anti-idiotype Antibody and Immunoassay Development Services

Upgraded! Anti-idiotype Antibody Services

Anti-Idiotype Antibodies

Features of GenScript ProBio Anti-ID Antibodies Service

Application tailored anti-id mAb

Fast delivery

High success rate

Anti-ID Package Details