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Protein Analytical Service

Protein Analytical Service

With world-class analytical techniques, GenScript is proud to provide a wide range of analytical testing services to ensure product quality and consistency during all stages of drug discovery and development. GenScript's experts have expertise to take both recombinant proteins and monoclonal antibodies, from research and product development through characterization and quality control tests. Services are available for both standalone analytical assays and broad testing programs, to meet your specific needs.

Key Features

  • Comprehensive breadth and innovative technologies
  • Flexible services, available for both standalone analytical assays and broad testing programs
Analytical services Analysis target Available method
Identification & characterization Protein identification Western Blot
Molecular weight LC-MS; MALDI-TOF MS
Peptide mapping RP-HPLC-UV
Sequence coverage LC-MS/MS
N-terminal/C-terminal sequence identification LC-MS/MS
Disulfide bond identification LC-MS/MS
N-glycosylation site identification LC-MS/MS
N-glycan analysis HILIC-HPLC/MS
PTM analysis LC-MS/MS
Higher order structure CD
pI cIEF; icIEF
Charge variants IEC-HPLC
Amino acid analysis RP-HPLC-UV/FLD
Purity & quantitation Purity SDS-PAGE; CE-SDS; iCE-SDS; SEC-HPLC
Quantitation UV; Bradford; Lowry; BCA; ELISA; AC-HPLC(protein A)
Impurity Host cell DNA(HCD) qPCR; SYBR green method
Host cell Protein(HCP) ELISA
Residual Protein A ELISA
Safety & Stability Bacterial Endotoxins Tal reagent
Microbial limit Membrane filtration
Sterile examination Membrane filtration
Abnormal toxicity  Animal test (mouse)
Mycoplasma PCR
Insoluble particles Light obscuration test
Visible particles Lamp test
Denaturation Temperature DSC

Case 1: Accurate MW Determination by LC-MS

  • Case sample: Antibody
  • LC method: RP-HPLC column, 28 min Gradient
  • MS mode: TOF MS
  • Data analysis: PeakView

Figure 1. Original mass spectrum

Figure 2. Deconvoluted mass spectrum

Figure 3. Deconvoluted mass spectrum after deglycosylation

Case 2: Glycan analysis by UHPLC-MS

  • Sample: Antibody
  • Sample type: solution in PBS
  • LC method: HILIC-HPLC column, 65 min Gradient
  • Scan Type: TOF MS
  • Reagent Kits from Waters


Figure 4. N-glycan types of antibodies (Trends in biotechnology, Volume 34, Issue 10, p835–846)


Figure 5. Total ion chromatogram of N-glycan by LC-MS method

Name Formula MW(labled) Charge Theoretical MW Experimental MW PPM RT(min) Intensity
A1   1424.6 +2H 713.3 713.3 0.3 12.11 41858
M3   1221.5 +2H 611. 611. 0.9 12.11 1630
F(6)A1   1570.6 +2H 786.33 786.33 0.6 13.5 114406
G0
C67H105O37N9
1627.7 +2H 814.84 814.84 0.8 14.02 2714
G0F
C73H115O41N9
1773.7 +2H 887.87 887.87 0 15.26 2E+06
G2-2SA
C101H159O63N11
2535 +2H 1268.5 1268.5 -3.2 15.29 101
M5   1545.6 +2H 773.81 773.81 0 16.51 101
G1a/G1b
C73H115O42N9
1789.7 +2H 895.86 895.86 -0.8 16.82/17.23 446
F(6)A1G(4)1   1732.7 +2H 867.35 867.35 0.5 16.85 56477
G0F-GN
C81H128O46N10
19.8 +2H 989.41 989.41 -1 17.54 4359
G1Fa/G1Fb
C79H125O46N9
1935.8 +2H 968.89 968.89 -0.6 17.95/18.42 6666
G2
C79H125O47N9
1951.8 +2H 9.89 9.89 -0.1 19.93 5205
G1F-GN
C87H138O51N10
2138.9 +2H 1070.4 1070.4 -0.6 20.19 40
G1F-SA
C90H142O54N10
2226.9 +2H 4.4 4.4 -0.5 20.27 29
G2F
C85H135O51N9
2097.8 +2H 1049.9 1049.9 -0.7 20.99 1898
G2-SA
C90H142O55N10
2242.9 +2H 1122.4 1122.4 -1.9 21. 1037
F(6)M4A1G(4)1Ga(3)1
  2056.8 +2H 1029.4 1029.4 2.1 22.23 647
G2F-GN
C93H148O56N10
2301.9 +2H 1152 1152 -0.5 22.94 583
G2F-SA
C96H152O59N10
2389.9 +2H 1196 1196 0.1 23.03 78
G2F-2SA
C107H169O67N11
2681 +2H 1341.5 1341.5 -0.7 25.26 3239


Figure 6. 20+ N-glycan types identified in the table



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