Cytokines are secreted proteins with various immune functions. Their main role is to regulate the immune responses of immune cells (i.e. pro-inflammatory or anti-inflammatory). Their expressions are tightly regulated in human body. Persistent and excessive production of inflammatory cytokines may cause autoimmune diseases or cancer. Antibodies targeting inflammatory cytokines have superior therapeutic benefits in pre-clinical research and clinical trials.
GenScript ProBio cytokine neutralization assay platform offers customized cytokine neutralization assays assessing cell viability and downstream signaling transduction for evaluating the biological activity of the antibody drugs at early drug discovery stage.
Case 1: anti-TNFα antibody neutralization assay
TNFα shows cytotoxic effect against L929 cell line
Humira is able to neutralize the activity of TNFα, cytotoxic effect is significantly reduced
Case 2: Anti-IL-6 neutralization assay
The neutralization assay focusing on TF-1 cell proliferation is not sensitive enough due to a small assay window. But if cell proliferation is changed to STAT3 phosphorylation level, the neutralization assay becomes more sensitive with an assay window more than 10 fold.
IL-6 stimulates the growth of TF-1 cells
Anti-IL-6 antibody is able to neutralize the effect of IL-6 to stimulate the growth of TF-1 cells
IL-6 stimulates STAT3 phosphorylation of TF-1 cells
Anti-IL-6 antibody is able to neutralize the effect of IL-6 to stimulate the STAT3 phosphorylation of TF-1 cells
The VEGF neutralization assay utilizes a VEGFR reporter gene assay cell line. The interaction between VEGF and VEGFR will activate the luciferase expression. When the interaction is disrupted, luminescence signal will decrease.