Characterizing biological therapeutics by optimized in vitro functional assays has been utilized to intermediately predict clinical outcomes and immunogenicity risks in a readily performed way. Peripheral blood mononuclear cells, isolated from human blood, consist of diverse immune cells, and are a powerful in vitro model in immunological research, biomarker identification and drug discovery. GenScript ProBio offers highly customized approaches to monitor the induction of immune responses with considerable performance characteristics of your biological candidates.
MoA of anti-immune checkpoint antibodies (Cancer Immunotherapy).
Case 1. Characterizations of OX40 antibody by T cell stimulation assay
OX40 receptors are co-stimulatory immune checkpoints on activated immune cells. Triggering OX40-mediated signaling pathways on T cells leads to enhanced cytokine production and T cell expansion.
Figure 1. Anti-OX40 antibody enhanced IFN-y production (left) and cell proliferation of CD4+ T cells (right) in a dose-dependent manner.
Case 2. Characterizations of PD-1 antibody by MLR assay
PD1/PDL1 pathway is a typical mechanism of tumor immune escape. PD1/PDL1 inhibitors boost anti-tumor immunity through recovering adaptive immune responses.
Figure 2. Anti-PD1 antibody enhanced IL-2 (left) and IFN-y (right) productions in MLR assay.