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sdAb Humanization Discovery Service Workflow

  • sdAb immunized Naïve Library Service

    Llama naive library
    Phage bio-panning for 2-3
    rounds and screening
    Antibody production and
  • SdAb Immunized Library Service

    Preparation of immunogen
    Llama/Camel immunization
    Phage display library construction
    Phage biopanning for 2-3 rounds and screening
    FASEBA HTS to identify best binders
    Antibody production and characterization

Service highlights

VHH SdAb Immune Library

  • Using non-immunized Alpaca/Llama for each project
  • 20+ projects delivered, 100% success rate

SdAb Naïve Library

  • Library Size: 1.02×1011 (300 donors)
  • Quality of Library
    • In frame rate/ORF rate: >95%
    • Diversity: Normal Distribution of CDR3 diversity
  • As fast as 2 weeks to deliver single domain antibody sequences

Diversified panning & screening strategies to ensure diversity & affinity

  • Panning:
    • Solid panning, liquid panning, cell panning, combination panning, competitive panning tailored to different type of targets
    • Up to 2 panning strategies are used to maximize the success rate
    • No more than 3-round panning to retain the sequence diversity
  • Screening:
    • FASEBA HTP screening combined with ELISA/FACS binding and SPR affinity ranking

Single Domain Antibody leads with high affinity (10-9~10-10 or better) generated in case study

SdAb Naïve Library Service

Milestone Items Deliverables
1 Phage display panning
  • Delivery: Up to 10 sequences
  • 0.5 mg/Ab purified antibodies (Optional)
2 ELISA or FACS Screening
3 sdAb expression & purification (Optional)
4 Additional sequence (Optional)
Total (no optional steps included): 2-3 weeks

SdAb Immunized Library Service

Milestone Items Deliverables
1 Llama/Alpaca immunization*
  • Delivery: All screened sequences
  • 0.5 mg/Ab purified antibodies (Optional)
2 sdAb phage display library construction(size of > 5 x 108)
3 Phage display panning
4 ELISA or FACS Screening
5 High throughput screening (Optional)
6 sdAb expression & purification (Optional)
Total (no optional steps included): 12-16 weeks

Please download service quotation form and fill it before sending to us.