Single Domain Antibody Discovery Services

150+ projects delivered, 95% success rate

Large size of naïve library (2×10¹¹) with high quality

By Dec 2022

The Status of sdAb humanization as Therapeutics

sdAb is a promising next-generation therapeutic antibody technology for cancer immunotherapy and other applications.

- Efficacy and safety validated in clinical studies
- Easy to modify for specific applications
- Relative-short discovery cycle
- Pursued by main-stream pharmaceutical companies
- Cost-effective in manufacturing

sdAb immunized Naïve Library Service

Llama naive library

Phage bio-panning for 2-3
rounds and screening

Antibody production and
characterization

SdAb Immunized Library Service

Preparation of immunogen

Llama/Camel immunization

Phage display library construction

Phage biopanning for 2-3 rounds and screening

FASEBA HTS to identify best binders

Antibody production and characterization

Outstanding track record:
- 150+ sdAb lead generation projects
- 100+ sdAb humanization projects, 100% success rate
- 6 global licensing deals
Fast timeline: 7 weeks to get humanized sdAb leads
2 lead generation campaigns available
- sdAb naïve library: 300 donors, 2×10¹¹ library size
- sdAb immunized library: using non-immunized Alpaca for each project
Specially designed mutation strategy for sdAb humanization to ensure normal expression of sdAb
PML based affinity maturation to improve the affinity at least by 10 folds
14 functional assay categories, 40+ ready-to-use assays for different targets

Milestone	Items	Deliverables
1	Phage display panning	Delivery: Up to 10 sequences 0.5 mg/Ab purified antibodies (Optional)
2	ELISA or FACS Screening
3	sdAb expression & purification (Optional)
4	Additional sequence (Optional)
Total (no optional steps included): 2-3 weeks

Milestone	Items	Deliverables
1	Llama/Alpaca immunization*	Delivery: All screened sequences 0.5 mg/Ab purified antibodies (Optional)
2	sdAb phage display library construction(size of > 5 x 10⁸)
3	Phage display panning
4	ELISA or FACS Screening
5	High throughput screening (Optional)
6	sdAb expression & purification (Optional)
Total (no optional steps included): 12-16 weeks

Please download service quotation form and fill it before sending to us.